Figure 2.

Intracellular Ca²⁺ transients activated by NMDA in rat TG neurons: (A) Representative Ca²⁺ traces recorded after glycine (30 µM) and NMDA (100 µM) applications to TG neurons. All agonists were in magnesium free solution. NMDA sensitive neuron also responded to the TRPV1 agonist capsaicin; (B) Histograms showing the percentage of neurons responding to glycine and NMDA in magnesium free solution vs. NMDA in basic solution (Mg 1 mM); (C) Representative Ca²⁺ traces recorded after NMDA (100 µM) and combination of DL-APV (80 µM) + NMDA applications to TG neurons. All drugs were in magnesium free solution. (D) Histograms showing the mean amplitude of Ca²⁺ -response activated by NMDA and against the background of DL-APV. Mean ± SEM. * p < 0.05.