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. 2022 Jan 29;23(3):1590. doi: 10.3390/ijms23031590

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of MHY2245 on SIRT and the expression of DNA damage response mediators and effector cascade in HCT116 cells. (A) Graph showing SIRT1 activity. Results are presented as mean ± SD, n = 3, and are expressed as a percentage of SIRT1 activity in vehicle-treated control cells. ** p < 0.01 and *** p < 0.001 compared with vehicle-treated control cells. (B) Cells were treated with the indicated concentrations of MHY2245 for 24 h and Western blot analyses were conducted to investigate the expression of SIRT1, SIRT2, Ac-p53 (Lys382), p53, γ-H2AX (Ser139), p-ATR (Ser428), p-ATM (Ser1981), p-BRCA1 (Ser1524), p-Chk1 (Ser345), and p-Chk2 (Thr68). β-actin was used as a loading control. Representative results from three independent experiments are shown.