Skip to main content
. 2022 Jan 29;23(3):1590. doi: 10.3390/ijms23031590

Figure 5.

Figure 5

Effect of MHY2245 on cell cycle modulation in HCT116 cells. (A) Exponentially growing cells at 60–70% confluence were treated with the indicated concentrations of MHY2245 for 24 h. Propidium iodide (PI)-stained cells were subjected to flow cytometry analysis to determine the cell distribution at each stage of the cell cycle. (B) The percentage of cells in the G0/G1, S, and G2/M phases of the cell cycle was calculated and is displayed on each bar graph. Results are presented as mean ± SD, n = 3, and are expressed as a percentage of that in vehicle-treated control cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with vehicle-treated control cells. (C) Effects of MHY2245 on the expression of cell cycle regulatory proteins analyzed via Western blot analysis of total lysates of cells treated with increasing concentrations of MHY2245. The membranes were probed with Cyclin B1, Cdc2, and Cdc25c. β-actin was used as a loading control. Representative results from three independent experiments are shown.