Figure 4.

Glucose deprivation–induced Akt phosphorylation is partially restored after the glucose re-addition. (A) Protocol of repetitive periods of glucose deprivation using a glucose-free medium and glucose re-addition using a conditioned medium (cM). Control cells were incubated in a complete growth medium. (B) Representative Western blot images and quantification of p-Akt levels in SH-SY5Y cells incubated as shown in (A), compared to control cells. Data are presented as mean ± S.E.M., n = 3, * p < 0.05, ** p < 0.01, † p < 0.001, compared to control. (C) Representative Western blot image of p-Akt in glucose-deprived SH-SY5Y cells (for 60 min) followed by glucose supplementation (1 mg/mL) for 5, 10, and 15 min.