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Oxidative stress is involved in LPC-induced apoptosis of mouse ovarian granulosa cells. The cells were treated with 160 μM LPC for 24 h in the presence or absence of 5 mM NAC, then the cell viability (A), the protein levels of cleaved caspase-8, cleaved caspase-3, Bax and Bcl-2 (B,C), and the number of annexin V-FITC-positive cells (D,E) were observed by CCK-8, Western blot and flow cytometry, respectively. * p < 0.05.
