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. 2022 Jan 27;23(3):1479. doi: 10.3390/ijms23031479

Figure 6.

Figure 6

Inhibition of autophagy alleviates LPC-induced apoptosis in mouse ovarian granulosa cells. Mouse ovarian granulosa cells were treated with 160 μM LPC for 24 h with or without 1 mM 3-MA, then the cell viability (A) and the protein levels of cleaved caspase-8, cleaved caspase-3, Bax and Bcl-2 (B) were detected by CCK-8 assay and Western blot, respectively. (C) Densitometry was used to quantify the relative protein levels. (D,E) Flow cytometry was used to count the number of annexin V-FITC(+)/PI(-) and annexin V-FITC(+)/PI(+) cells. * p < 0.05.