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. 2022 Jan 22;23(3):1223. doi: 10.3390/ijms23031223

Figure 6.

Figure 6

A higher fraction of TR-bound genes is regulated in the hind limb of wild-type tadpoles by T3 treatment than those in TRα (-/-) tadpoles. (A) Venn diagram showing the overlap between TR-bound genes identified by ChIP-Seq in WT hind limb and T3-upregulated genes in WT hind limb. Of the 3714 TR-bound genes identified by ChIP-seq, 24% were shown as the T3-upregulated genes. Conversely, 25% of the T3-upregulated genes were found to be TR-bound. (B) Venn diagram showing the overlap between the TR-bound genes identified by ChIP-seq in WT hind limb and T3-downregulated genes in WT hind limb. Of the 3714 TR-bound genes identified by ChIP-seq, 19% were shown as the T3-downregulated genes. Conversely, 19% of the T3-downregulated genes were found to be TR-bound. (C) Venn diagram showing the overlap between TR-bound genes identified by ChIP-seq in TRα (-/-) hind limb and T3-upregulated genes in the TRα (-/-) hind limb. Of the 2499 TR-bound genes identified by ChIP-seq, 7% were shown as T3-upregulated genes. Conversely, 17% of the T3-upregulated genes were found to be TR-bound. (D) Venn diagram showing the overlap between the TR-bound genes identified by ChIP-seq in TRα (-/-) hind limb and T3-downregulated genes in TRα (-/-) hind limb. Of the 2499 TR-bound genes identified by ChIP-seq, 7% were shown as T3-downregulated genes. Conversely, 12% of the T3-downregulated genes were found to be TR-bound. (E) Venn diagram comparison of the 1407 genes bound by TR only in wild-type hind limb to the 899 genes upregulated by T3 and bound by TR in the WT hind limb. Note that 24% of the genes bound by TR only in wild-type hind limb were upregulated by T3, similar to the percentage of upregulated genes among all genes bound by TR in WT hind limb (A). (F) Venn diagram comparison between 2307 genes bound by TR in both wild type and TRα (-/-) hind limb and the 899 genes upregulated by T3 and bound by TR in WT hind limb. Note that 24% of the genes bound by TR in both wild type and TRα (-/-) hind limb were upregulated by T3, similar to the percentage of upregulated genes among all genes bound by TR in WT hind limb (A). Thus, the data in A, E, F indicate that genes bound by TR in either wild type only or both wild type and TRα (-/-) hind limb are similarly regulated by T3 treatment of wild type premetamorphic tadpoles.