Table 1.
Vasodilatory effects of NAGly, Abn-CBD, PSB-MZ-1415, and PSB-MZ-1440 on U46619 (0.01 µM)-precontracted isolated human pulmonary arteries (pEC25, pEC50), interaction with PSB-CB-27 and O-1918, and effect of endothelial removal.
| Group | n | Contraction (mN) | pEC25 | pEC50 | Rmax (%) |
|---|---|---|---|---|---|
| NAGly | 13 | 10.8 ± 2.5 | 5.8 ± 0.1 | 5.1 ± 0.1 | 92.5 ± 5.6 |
| +PSB-CB-27 (1 μM) | 7 | 9.9 ± 1.3 | 5.2 ± 0.1 ***a | N.D. | N.D. |
| +PSB-CB-27 (10 μM) | 7 | 10.5 ± 1.8 | 4.9 ± 0.1 ***a | N.D. | N.D. |
| +O-1918 (10 μM) | 7 | 9.8 ± 2.1 | 4.7 ± 0.1 ***a | N.D. | N.D. |
| Abn-CBD | 5 | 10.6 ± 2.5 | 6.2 ± 0.2 | 5.5 ± 0.1 | 97.4 ± 4.1 |
| +PSB-CB-27 (10 μM) | 7 | 9.7 ± 1.9 | 4.8 ± 0.1 ***b | N.D. | N.D. |
| Ethanol | 7 | 8.9 ± 2.6 | N.D. | N.D. | 4.8 ± 4.1 ***b |
| PSB-MZ-1415 | 10 | 9.9 ± 1.2 | 5.8 ± 0.2 | 5.2 ± 0.2 | 100.3 ± 5.9 |
| +PSB-CB-27 (10 μM) | 6 | 10.2 ± 1.3 | 4.6 ± 0.1 ***a | N.D. | N.D. |
| +O-1918 (10 μM) | 6 | 9.8 ± 1.8 | 4.9 ± 0.2 **a | N.D. | N.D. |
| +Endothelium | 3 | 10.3 ± 1.1 | 5.9 ± 0.1 | 5.0 ± 0.1 | N.D. |
| −Endothelium | 3 | 11.3 ± 1.2 | 4.8 ± 0.1 **b | N.D. | N.D. |
| PSB-MZ-1440 | 8 | 10.3 ± 2.0 | 6.2 ± 0.2 | 4.9 ± 0.1 | 106.5 ± 5.3 |
| +PSB-CB-27 (10 μM) | 6 | 9.5 ± 1.2 | 4.8 ± 0.1 ***b | N.D. | N.D. |
| DMSO | 10 | 10.5 ± 1.8 | N.D. | N.D. | 4.9 ± 4.0 ***b |
Data are expressed as the mean ± SEM and represent contractile responses to U46619 (0.01 µM). n, number of patients; ** p < 0.01, *** p < 0.001; compared to the respective control as determined by a one-way ANOVA followed by Dunnett’s post hoc test or b Student’s t-test. N.D., not determined.