Figure 1.

A schematic drawing illustrating the experimental protocol for the two separate series of male Brattleboro rats treated with bilateral AVP-AAV in the SON. (A) Series 1 and (B) Series 2. Colour code for the manipulations is as follows: blue, stressors and endocrine measures (ACTH and corticosterone); red, surgical procedures/treatments; green, behavioural testing; yellow, preparation for tissue collection. Abbreviations: ACTH, adrenocorticotropin; AVP-AAV, vasopressin-containing adeno-associated virus vector; EW, egg white injection; ISH, in situ hybridization; NOR, novel object recognition; RT-PCR, polymerase chain reaction; RI, resident intruder test; SON, supraoptic nucleus. For more details, see the text in Material & Methods.