Figure 1.
Effect of HP-NAP on melanoma growth in zebrafish model. (A) Scheme of the experimental design. M121224, RPMI-7951 or A375 human melanoma cells were stained with VybrantTM DiI, xenotransplanted into the yolk of transparent zebrafish larvae at 2 days post-fertilization (2 dpf)/0 days post-injection (0 dpi). At 4 dpf/2 dpi, embryos were injected with HP-NAP or saline (vehicle) as a negative control, and were observed at 9 dpf/7 dpi. (B,D,F) Representative fluorescence stereoscope images of embryos injected with M121224, RPMI-7951 and A375 cells respectively and treated with HP-NAP or vehicle. Arrowheads indicate metastases. Scale bar: 500 µm. (C,E,G) Scatter plots show the quantification of the tumor size (AU: Arbitrary Unit) at 9 dpf/7 dpi. Values are expressed as mean ± SEM and analyzed by Student’s t test; *, p < 0.05 and ***, p < 0.001; n = 7 for M121224 cells and n = 6 for RPMI-7951 and A375 cells (for each vehicle and HP-NAP treatment).