Figure 4.

Autophagy inhibition restores TRPM7 and MagT1 expression during myogenesis. C2C12 were cultured in CM or DM for 72 h in the presence of the proteasome inhibitor MG132 (0.25 µM) (a) or calpeptin (50 µM) (b) or bafilomycin (2.5 nM) (c). TRPM7, MagT1, MHC and Myomixer expression was analyzed by western blot. Vinculin was used as control of loading. A representative blot (left panel) and densitometry performed on three independent experiments and obtained by ImageLab (right panel) are shown. To define statistical significance, non-parametric Kruskal–Wallis test was used and the p-values were corrected using the Dunn’s method. * p ≤ 0.05.