Figure 6.

Silencing of Sirt1 or PGC-1α diminishes the protective effects of galangin under H₂O₂ exposure in HS68 cells. (A) Levels of Nrf2, p-Nrf2, and HO-1 measured by Western blot in response to Sirt1 and PGC-1α silencing. (B) After treatment, cells were stained using an SA-β-Gal staining kit to detect SA-β-Gal activity. Positive cells were stained in green, which indicates senescent cells. Values shown are means ± SE. Quantification of the results is shown (n = 3) * p < 0.05, ** p < 0.01, vs. galangin plus H₂O₂-treated cells.