Figure 2.

Intracellular concentration of reactive species in MCF7 (a,c,e) and HCC1806 (b,d,f) breast cancer cell lines 2 and 24 h after exposure to cold atmospheric plasma (CAP). Intracellular content of peroxide (a,b), superoxide anion (c,d) and nitric oxide (e,f) were assessed by fluorescence using DCH2-DA, DHE and DAF-FM diacetate probes, respectively. Results are presented normalized to the value 100% corresponding to control cell cultures not exposed to CAP. Data express the mean ±SE of at least two independent experiments per cell line. Statistically significant differences are shown with * p < 0.05, ** p < 0.01 and *** p < 0.001.