Figure 3.

PTK7^V354M variant increases cell proliferation, migration, and invasion. (A) Western blot results represent enhanced protein expression of PTK7 in HT29-PTK7^V354M cells compared to HT29-PTK7^WT cells. (B) Proliferation assays show significantly increased viable cell numbers of HT29-PTK7^V354M compared to HT29-PTK7^WT cells. Significance levels are included for each time point. ns—no significance, *** p < 0.001. (C) Representative image of migrated HT-29 cells after overexpression of PTK7^WT and PTK7^V354M. (D) Representative image of invaded HT-29 cells after overexpression of PTK7^WT and PTK7^V354M. * p < 0.05. PTK7^V354M–PTK7 plasmid with the mutation V354M, PTK7^WT–wild type PTK7 plasmid, pcDNA3–control vector.