Figure 4.

Effect of PTK7^V354M variant on downstream targets and cell cycle. (A) qPCR resulted in significant down-regulation of p53, p21, and CREB mRNA levels in HT29-PTK7^V354M cells. * p < 0.05, *** p < 0.01. (B) Western blot results representing enhanced protein expression of pAKT as well as decreased CREB, p53 and p21 protein expression in HT29-PTK7^V354M cells compared to HT29-PTK7^WT cells. (C) HT29-PTK7 ^V354M transfected cells resulted in significant cell cycle progression with less accumulation of cells in the G0/G1 phase compared to HT29-PTK7^WT cells and a concomitant increase in G2/M phase, confirming the cell proliferation results. ns—no significance, * p < 0.05. (D) HT29-PTK7 ^V354M transfected cells showed upregulated CCND1 and CCNE protein expression, involved in cell cycle regulation. PTK7^V354M–PTK7 plasmid with the mutation V354M, PTK7^WT–wild type PTK7 plasmid, pcDNA3–control vector.