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. 2022 Jan 27;23(3):1428. doi: 10.3390/ijms23031428

Figure 5.

Figure 5

High Na+ environment regulated NKCC-1 signaling pathway in lymphatic endothelial cells (LECs). (A) Cultured LECs exposed to a high-sodium environment showed greater expression of NKCC1 mRNA, (B) while expression of phosphorated NKCC-1 protein decreased. (C) High-sodium environment decreased protein expression of SPAK, an upstream activating kinase of NKCC1. Experiments were performed independently 3 times using 3 wells per treatment and analyzed by ANOVA followed by Dunnett multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.