Figure 6.
Identification of target proteins regulated by the miRNA landscape of shear stress-induced EC-sEVs. (A) Protein–protein interaction network obtained using STRING software in shear stress-induced EC-sEVs to experimentally identify important protein nodes in predicted target proteins. (B) HUVECs were treated with LSS- or OSS-sEVs (3 × 109 particles) for 24 h. The protein levels of VEGFA, PTEN, ROCK1, HIF1A, IL-6, EGFR, ESR1, IGF1R, BCL2, TGFBR2, and NOTCH1 were determined by Western blotting. The protein expression levels were normalized to GAPDH (internal control). Representative images from at least five experiments are shown (n = 5; * p < 0.05 compared to LSS-sEVs). Mean ± SEM. Statistical analysis was performed using the nonparametric Mann–Whitney U test.