Figure 4.

Increased expression of bglG affects only IS insertions into the bgl operon but not the fucAO, glpFK and flhDC operons. (A) Effect of bglG expression on PPD⁺ mutations. A total of 10⁸ cells were applied onto M9 + PPD plates, and PPD⁺ colonies were counted over time. (B) Effect of bglG expression on Glp⁺ mutations. A total of 2 × 10⁷ cells of a crp deletion strain with or without Ptet driving bglG were applied onto M9 + glycerol plates, and Glp⁺ colonies were counted over time. (C) Effect of bglG expression on swarming mutations. A total of 2 × 2 μL of cell suspensions with an OD₆₀₀ of 1 were streaked over the surface of LB + 0.3% agar. Swarming mutants (as super swarming subpopulations) were counted per streak after 20 and 36 h of incubation at 30 °C. (D) Effect of bglG expression on PPD⁺ mutations in the presence of salicin. A total of 10⁸ cells of strains ΔbglB and ΔbglB_Ptet-G were individually applied to M9 + PPD + salicin (0.5%) plates. (E) Effect of bglG expression on Glp⁺ mutations in the presence of salicin. A total of 2 × 10⁷ cells of strains Δcrp and Δcrp_Ptet-G were individually applied to M9 + glycerol + salicin (0.5%) plates. (F) Effect of bglG expression on swarming mutations in the presence of salicin. A total of 2 × 2 μL of cell suspensions with an OD₆₀₀ of 1 were streaked over the surface of LB+ salicin + 0.3% agar.