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. 2022 Jan 28;23(3):1505. doi: 10.3390/ijms23031505

Figure 6.

Figure 6

Effects of altering the BglG binding site on the nascent mRNA on the frequency of Bgl+ mutations. (A) Diagram of the first terminator T1, a Rho-independent hairpin structure. The BglG binding sequences (OBglG), partially overlapping with the hairpin structure, are bolded. (B) Diagram of the first terminator with a 2-bp (UG) deletion in the BglG binding site on the mRNA. In both A and B, the transcriptional start site is labeled +1. The RBS is underlined, and the start codon, ATG, of bglG is bolded. (C) Effect of deleting the “UG” bases in OBglG (referred to as strain “OBglGMut”) on Bgl+ mutation frequencies in wild type cells. (D) Effect of deleting the “UG” bases in OBglG on Bgl+ mutations in Ptet-G cells (that is, strain OBglGMut_Ptet-G). (E) Effects of various BglG levels on Bgl+ mutation frequencies in OBglGMut cells (that is, strain R_ OBglGMut_Ptet-G) with an altered BglG binding site in the DNA and the nascent mRNA.