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. 2022 Jan 29;23(3):1582. doi: 10.3390/ijms23031582

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of eupatilin on inflammatory cytokines in LPS-stimulated RAW264.7 cells. RAW264.7 cells were induced with 1 µg/mL LPS and treated with various concentrations (10, 25, 50, and 100 µmol/L) of eupatilin for 24 h. (a) Cell viability after treatment with different concentrations of eupatilin for 24 h. (b) NO levels in cell supernatants after LPS and eupatilin treatment. (c) IL-6 levels in cell supernatants after LPS and eupatilin treatment. (d) ROS levels in RAW264.7 cells treated with LPS and eupatilin. 630× magnification; scale bar: 25 μm. Data represent the mean ± SEM (n = 4). #### p < 0.0001 vs. control group; * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. LPS group.