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. 2022 Feb 4;23(3):1773. doi: 10.3390/ijms23031773

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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AHR activation and induction of epidermal differentiation in human epidermal equivalents. (A) H&E staining of human epidermal equivalents (HEEs) stimulated with 1 nM of IMA-compounds and TCDD for 96 h (re-stimulated after 48 h). Double headed arrows in magnified images indicate stratum granulosum layers (green arrows show an increase compared to the control-HEE). (B) CYP1A1 mRNA levels (n = 2), mean +/− SEM. (C) Immunostaining for filaggrin (FLG) after 96 h of stimulation with IMA-compounds. Scale bar = 100 µm.