Figure 2.

Nanofiber–film hybrid system facilitates unidirectional release of desmopressin. (a) Experimental setup used for studying the release of desmopressin from the nanofibers. The release of desmopressin into both chambers (in the Ussing chamber setup) separated either by the nanofibers or by the nanofiber–film hybrid system was determined. (b) Release of desmopressin from nanofibers or the nanofiber–film hybrid system in the left (blue) and right (red) Ussing chamber, respectively, as depicted in (a). The cumulative release was based on a loading of 8% (w/w) desmopressin in the chitosan/PEO nanofibers. The data are presented as mean ± SD. N = 3, n = 2–3, where N represent the number of individually prepared nanofibers/hybrid systems and n is the number of measurements per system.