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. 2022 Jan 25;23(3):1358. doi: 10.3390/ijms23031358

Figure 4.

Figure 4

Effect of calycosin on the melanogenesis-related protein expression levels in B16F10 cells. Western blot analyses of the dose-dependent effect of calycosin on the expressions and phosphorylations of (A) CREB, p38, JNK, and (B) ERK and Akt in B16F10 cells treated with 0.2 μM α-MSH and indicated concentrations of calycosin for 48 h. (C) Western blot analyses of the time-dependent effect of calycosin on the expressions and phosphorylations of ERK and Akt in B16F10 cells treated with 40 μM or 80 μM calycosin for indicated time periods. (D) Western blot analysis of the dose-dependent effect of calycosin on the expression level of β-Catenin in B16F10 cells treated with 0.2 μM α-MSH and indicated concentrations of calycosin for 48 h.