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. 2022 Feb 1;23(3):1700. doi: 10.3390/ijms23031700

Figure 1.

Figure 1

Pharmacological inhibition of Akt by triciribine leads to an increased INa current density in HEK-293 cells expressing Nav1.6. (A) Representative traces of INa currents recorded from HEK-Nav1.6 cells treated for 60 min either with DMSO (0.1%) (black) or 30 µM triciribine (red). (B) Current–voltage relationship showing increased peak INa current densities in triciribine-treated HEK-Nav1.6 cells (red, n = 11) compared to DMSO control (black, n = 11). (C) Peak current density at voltage step of −10 mV in DMSO and triciribine-treated HEK-Nav1.6 cells. A 60 min treatment with triciribine (30 µM) significantly increased the Nav1.6-mediated peak INa current density (n = 11 in both group; ** p = 0.0076 with Student’s unpaired t-test). (D,E). Voltage dependence of INa activation (D) and steady-state inactivation (E) in DMSO and triciribine-treated HEK-Nav1.6 cells. Treatment with triciribine did not affect voltage dependence of INa activation or steady-state inactivation. Inset shows the inactivation protocol (scale bars: 20 mv/100 ms). Box plot shows: Range (Perc 25, 75), Mean, Median, Min, and Max. Results are summarized in Table 1.