Figure 6.

Responsiveness of the HTR2B gene promoter to IL-4 and IL-6 in transfected T108 cells: (A) Representation of the HTR2B/CAT recombinant plasmids used for transfection analyses. Numbers indicate the position relative to the HTR2B theoretical mRNA start site. The position of the three conserved STAT sites is indicated (STAT-982, STAT-942 and STAT-280) (B) CAT activities measured following transfection of the HTR2B constructs shown in panel B in the UM cell line T108. CAT activity is expressed relative to the level directed by the HTR2B/-2000 construct. *: Values considered to be statistically significant from those obtained with the HTR2B/-138 construct (p value < 0.01). Addition of IL-4 and IL-6 increased CAT activity encoded by the plasmid HTR2B/-2000(WT_STAT-280) but not when the −280 STAT site is mutated in HTR2B/-2000(MU_STAT-280).