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. 2022 Feb 3;23(3):1758. doi: 10.3390/ijms23031758

Figure 1.

Figure 1

Decellularized MSC-derived ECM obtained after culturing the MSC for 2 days on standard tissue culture plates (ECM), on Matrigel-coated tissue culture plates (Matrigel-ECM), or on standard tissue culture plates with TGFβ1-supplemented medium (TGFβ1-induced ECM). Alcian blue staining was weak, suggesting low glycosaminoglycan content of the MSC-derived ECM, while picrosirius red staining was more distinct, demonstrating the presence of collagens. Note the fibrous structure of the TGFβ1-induced ECM.