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. 2022 Feb 3;23(3):1758. doi: 10.3390/ijms23031758

Figure 4.

Figure 4

Matrix metalloproteinases (MMP) in cell culture supernatants. (A): Total matrix metalloproteinase (MMP) activity, measured in the supernatants of MSC after culture on the different MSC-derived ECM substrates (ECM, Matrigel-ECM, TGFβ1-ECM) or in the respective control conditions without MSC-derived ECM (tissue culture plastic (TCP), Matrigel). MMP activity differed significantly between groups designated with the same letter (p ≤ 0.007 in the post hoc tests). (B): Representative image of gelatin zymography of the supernatants; bands within the same kDa range were detected in all samples, but with different intensity (arrows); ladder: Precision Plus Protein Dual Color Standards (Bio-Rad, Hercules, CA, USA); standard 1: 0.5 µg collagenase V; standard 2: 1 µg collagenase V.