Figure 5.

miR-9820-5p promotes pGCs apoptosis by targeting SRSF1. (A,B) The binding of miR-9820-5p and SRSF1-WT was predicted by RNAhybrid and verified by dual-luciferase activity analysis. (C,D) SRSF1 mRNA and protein levels after transfection of miR-9820-5p mimics. (E,F) SRSF1 mRNA and protein levels after transfection of miR-9820-5p inhibitors. (G) The shift of GC apoptosis rates after co-transfection of miR-9820-5p inhibitors and si-SRSF1 detected by flow cytometry. Data are expressed as the mean ± SEM of three experiments; * p < 0.05, ** p < 0.01.