Evaluation of the coding ability of circSLC41A1. (A) Upper panel: the putative open reading frame (ORF) in circSLC41A1. Note that the circSLC41A1 junction is inside the ORF1. Lower panel: the sequences of the putative ORF are shown in green, internal ribosomal entrance site (IRES) sequences are shown in orange, the potential m6A site is shown in red, and specific amino acid sequences of ORF1 are shown in purple. (B) Hydrophobicity analysis of circSLC41A1-134aa. The ordinate score greater than 0 indicates hydrophobicity and less than 0 indicates hydrophilicity. (C) The transmembrane domain prediction of circSLC41A1-134aa. The transmembrane domain is shown in red, the intramembranous region is shown in blue, and the extramembranous region is shown in fuchsia. (D) The tertiary structure prediction of circSLC41A1-134aa. α-helix, β-sheet, and a random coil are shown in red, blue, and yellow, respectively. (E) The ligand prediction of 134aa. The green segments represent ligands and red segments represent the binding domains.