Table 1.

Examples of the seed endosperm accumulation of recombinant pharmaceutical proteins in stable transgenic cereal plants.

Recombinant Protein 1	Origin	Host Plant	Yield	References
Derivatives of Japanese cedar pollen allergens Cry j 1 and Cry j 2	Cryptomeria japanica	O. sativa	Up to 60 µg/seed	[61,62,63]
Derivatives of house dust mite allergens	Dermatophagoides spp.	O. sativa	Up to 90 µg/seed	[64,65,66]
Birch pollen allergens	Birch	O. sativa	Up to 550 µg/seed	[67,68]
LTB–COE	PEDV	O. sativa	1.3% of endosperm TSP	[69]
CTB	Vibrio cholerae	O. sativa	Up to 30 µg/seed	[70]
CTB-vaccine antigens (As14 and As16)	Ascaris suum	O. sativa	50 µg/g seed, 1.5 µg/seed	[71,72]
Chimeric HBV surface antigen SS1	HBV	O. sativa	31.5 ng/g seed	[73]
AMP Cecropin A	Insects	O. sativa	Up to 6 μg/g seed	[74]
IL-10	Human	O. sativa	1.2 mg/g seed, 219 μg/seed	[75,76]
Anti-HIV mAb 2G12	Human	Z. mays	Up to 60 µg/g seed	[58]
Anti-HIV mAb 2G12	Human	H. vulgare	160 μg/g seed	[59]
Cathelicidin AMP LL-37	Human	H. vulgare	Up to 550 μg/kg seed	[60,77]

¹ Abbreviations: LTB-COE, a fusion protein composed of the B-subunit of the E. coli heat-labile enterotoxin (LTB) and a synthetic core-neutralizing epitope (COE) of porcine epidemic diarrhea virus (PEDV); CTB, cholera toxin B subunit; HBV, Hepatitis B virus; AMP, antimicrobial peptide; IL, interleukin; HIV, human immunodeficiency virus; mAb, monoclonal antibody.