Figure 3.

Effect of C-phycocyanin on cytokine production from cortical microglia. Microglia were cultured overnight in medium containing 10% of serum, which was replaced with serum-free medium before treatment with 100 μg/mL C-PC, 100 ng/mL LPS, or their association for 6 h. IL-1β (A) and TNF-α (B) mRNA levels were quantified by real-time PCR. Data are means ± SEM (n = 3 in triplicate). * p ˂ 0.05 and ** p ˂ 0.01 versus control cells (CTR). One-way ANOVA followed by Holm–Sidak’s test.