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. 2022 Jan 27;23(3):1440. doi: 10.3390/ijms23031440

Figure 5.

Figure 5

Effect of polymyxin B on cytokine release from cortical microglia. Microglia were cultured overnight in medium containing 10% of serum, which was replaced with serum-free medium before pretreatment with 50 μg/mL polymyxin B (PMB) for 1 h followed by stimulation with LPS (100 ng/mL) or C-PC (100 μg/mL) for 16 h. Supernatant were collected and analyzed for IL-1β (A) and TNF-α (B) content. Data are means ± SEM (n = 4 in triplicate). * p ˂ 0.05 and *** p ˂ 0.001 versus control cells. °°° p ˂ 0.001 versus LPS treatment (black bars). One-way ANOVA followed by Holm–Sidak’s test.