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. 2022 Feb 3;23(3):1762. doi: 10.3390/ijms23031762

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enhancing effect of OPAC on the total ALDH activity in hepatoma cells. (A) Chemical structures of OPAC and its related compounds, such as protocatechuic acid, benzoic acid, and hexanoic acid. (B) Effect of OPAC on cell viability. Hepa1c1c7 cells were treated with the indicated concentrations of OPAC for 6 h. Cell viability was measured using an MTT assay. (C–F) Modulating effect of OPAC and its related compounds on the total ALDH activity in mouse Hepa1c1c7 cells. Hepa1c1c7 cells were treated with the indicated concentrations of OPAC (C), protocatechuic acid (D), benzoic acid (E), and hexanoic acid (F) for 6 h. The total ALDH activity was determined by a microplate reader (340 nm). The values are expressed as the ratio of the control pretreated only with DMSO. (G) Enhancing effect of OPAC on the total ALDH activity in human HepG2 cells. HepG2 cells were treated with the indicated concentrations of OPAC for 6 h. The total ALDH activity was determined by a microplate reader (340 nm). The values are expressed as the ratio of the control pretreated only with DMSO. All values are means ± SD of three independent experiments. *p < 0.05 vs. control.