Figure 2.

Modulating effects of OPAC on the gene and protein expressions of the classical family of ALDHs: ALDH1A1, ALDH2, and ALDH3A1. (A) The total RNA was extracted from the Hepa1c1c7 cells treated with OPAC for 6 h; then, an RT-PCR analysis for each gene was carried out. Representative blots and quantitative data for ADLH1A1, ADLH2, and ADLH3A1 are shown. (B) Hepa1c1c7 cells were treated with OPAC for 6 h, and the total cell lysates were subjected to a Western blot analysis. All values are expressed as means ± SD of three separate experiments. *p < 0.05 vs. control.