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. 2022 Feb 1;16(2):e0009926. doi: 10.1371/journal.pntd.0009926

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© 2022 Glockzin et al

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Fig 6 — SDS-PAGE analysis of CNBr cleavage reaction (A) and Native-MS of recombinant APRT2s (B, C). (A) When APRT2 methionine residues are not oxidized to MetO, bands of molecular mass with the expected sizes indicated in S14 Fig would be observed. APRT2 expressed in E. coli and stored in the presence of 5 mM DTT (APRT2 Ec) was used as a control. The bands resulting from CNBr cleavage for the APRT2-Ntag, APRT2-Ctag, and APRT2 Ec are shown in the (+) lanes. Negative controls (-) and untreated native protein (o) show the band corresponding to the monomer mass. Native-MS of recombinant APRT2-Ntag (B) and APRT2-Ctag (C) provided the measured masses of 25,860 and 26,637 Da, respectively.