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| Enzyme | Variable substrate | Fixed substrate | Apparent kinetic parametersa | ||
|---|---|---|---|---|---|
| KM (μM) | kcat (s-1) | kcat/KM (× 103 M-1 s-1) | |||
| APRT1 | Adenine (1.5–150 μM) | PRPP (1 mM) | 3.7 ± 0.5 | 1.74 ± 0.03 | 470 ± 64 |
| APRT2 | Adenine (50–500 μM) | PRPP (1 mM) | 253 ± 4 | 0.003 ± 0.0002 | 0.012 ± 0.0008 |
aInitial velocity data were fit to Eq (1) to afford apparent kinetic parameters
