Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 8;23(3):1904. doi: 10.3390/ijms23031904

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of the TST and foot shock on P2X7R-immunoreactivities (IRs), with microglial (Iba1), astroglial (GFAP), and (pro)oligodendroglial (Olig2, NG2) markers as well as their co-localization with 4’,6-diamidino-2-phenylindol (DAPI) in the hippocampal CA1 region of 3-week old rats. (A) fluorescence microscopic pictures of immunopositive cells and DAPI-labelled nuclei. The astrocytic marker glial fibrillary acidic protein (GFAP; green fluorescence)-IR co-stains with the red fluorescent P2X7R-IR, and the blue fluorescent DAPI. Triple-labelling of all three IRs is displayed in the right row of the picture as shown. One representative snapshot obtained from the hippocampi of 8 animals. Scale bars, 100 µm. (B–F) The stained sections were examined under 400× magnification of the microscope over the whole image; the IF was evaluated with ImageJ software and, after subtracting the background, it was expressed as a % of the whole area. (B,C) the Iba1/P2X7-IR was increased by the TST and foot shock both with and without minocycline pretreatment of mice (see Section 4). However, minocycline largely decreased the number and density of the microglial cells. (E,F) oligodendrocytes and NG2 glial cells responded to the TST and foot shock in a similar manner as microglia did. (D) neither the GFAP- nor the GFAP/P2X7R-IR changed after TST or foot shock stimulation in comparison with the unstressed controls. * p < 0.05; statistically significant difference from the first column in a triad of columns in each panel (B, F = 2.635, p = 0.090; C, F = 9.681, TST, p = 0.002, foot shock, p = 0.005; D, F = 0.751, p = 0.484; E, F = 20.661, TST, p < 0.001, foot shock, p < 0.001; F, F = 4.258, TST, p = 0.093, foot shock, p = 0.032). ⁺ p < 0.05; statistically significant difference from the second column in a triad of columns in each panel (B, F = 6.206, TST, p = 0.016, foot shock, p = 0.010; C, F = 7.581, TST, p = 0.009, foot shock, p = 0.006; D, F = 3.265, p = 0.058; E, F = 41.398, TST, p < 0.001, foot shock, p < 0.001). One-way ANOVA, followed by the Holm–Sidak test in each case. The number of experiments is indicated in the first set of columns.