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. Author manuscript; available in PMC: 2022 Feb 11.
Published in final edited form as: Methods Mol Biol. 2021;2215:49–82. doi: 10.1007/978-1-0716-0966-8_3

Table 2:

Typical scan settings for SEM and FIB views for frozen hydrated specimens. ‘Live scan’ and ‘snapshot’ are used to monitor lamella milling. The ‘photo’ preset is typically used to generate a low magnification grid overview due to the high electron dose. Snapshots and photos are not used in FIB view due to the potential for beam damage.

Live (FIB/SEM) Snapshot (SEM) Photo (SEM)
Dwell Time 200 ns 200 ns 2 μs
Line Integration 1 1 1
Resolution 1536 × 1024 3072 × 2048 6144 × 4096
Bit Depth 8 8 8–16