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. 2022 Feb 4;23(3):1788. doi: 10.3390/ijms23031788

Figure 2.

Figure 2

EZH2 affects the activation of the cGAS-STING pathway through CCF in breast cancer cells. Western blotting was used to detect the P-STING, STING, and IL-6 level in MM-231-shCtrl/MM-231-shEZH2 cells (A), MM-231 cells treated with EPZ-6438 (1 μM) for 72 h (B), MM-231 cells treated with cGAMP (5 μM) for 4 h (C), MCF-7-EZH2 cells (D), MCF-7-EZH2 cells treated with the CCF inhibitor CCCP (E), MCF-7-EZH2 cells overexpressing MCAK (F), MM-231-dnMCAK cells treated with EPZ-6438 (1 μM) for 72 h (G). (H–I) MM-231 cells were immunofluorescent stained with DAPI, H3K27me3, EZH2, and cGAS to calculate the ratio and observe cGAS activated by CCF (white arrow) or naked DNA (yellow arrow). Meanwhile, we observe whether there is EZH2 in the activated cGAS, scale bar = 5 μm. Each experiment was repeated at least three times. Error bars, mean ± SD, ***, p < 0.001.