Skip to main content
. 2022 Feb 6;11(3):851. doi: 10.3390/jcm11030851

Figure 1.

Figure 1

Effect of CCNDBP1 expression on HCC cell growth. The development of CCNDBP1-overexpressing cell lines and the cell growth of hepatocellular carcinoma (HCC) cell lines and the permanent clones overexpressing CCNDBP1 were determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (a) Representative reverse transcription polymerase chain reaction (RT-PCR) of CCNDBP1 and glyceraldehyde 3 phosphate dehydrogenase (GAPDH) and Western blotting (WB) of CCNDBP1 and β-actin are shown. (b,c) Growth of cell lines in normal culture condition with 10% fetal bovine serum. (d,e) Cell growth after irradiation with 0.8 Gy of X-ray. (f,g) Cell growth with 20 µM of CDDP. The values represent mean ± standard deviation (n = 3 for each group at the time points). * p < 0.05 and no statistical significance (NS) on two-way analysis of variance followed by Bonferroni’s multiple comparison test.