Figure 2.

Effect of proximal tubule (PT)-specific Na⁺-bicarbonate cotransporter, electrogenic, isoform 1 (NBCe1) deletion [PT-NBCe1-A/B knockout (KO)] on serum and urine chemistries. A: serum K⁺ measurement. PT-NBCe1-A/B KO did not alter serum K⁺ significantly. B: serum ${\text{HCO}}_3^{-}$ measurement, demonstrating that serum ${\text{HCO}}_3^{-}$ was significantly lower in KO mice than in wild-type (WT) mice. C: urine ammonia excretion, calculated as the average of two consecutive 24-h urine collections. PT-NBCe1-A/B KO did not alter urine ammonia excretion significantly. D: urine pH calculated as the average pH of two consecutive 24-h urine collections. PT-NBCe1-A/B KO significantly decreased urine pH. n = 4 male (M)/3 female (F) WT mice and 5M/4F KO mice for serum K⁺ and serum ${\text{HCO}}_3^{-}$. n = 5M/4F mice of each genotype for urine ammonia excretion and urine pH. Statistical analysis used ANOVA with genotype and sex as independent variables. Sex did not significantly alter the effects of genotype on any of the reported analyses. NS, not significant.