Figure 1.

Depletion of PF4, histones/nucleosomes and HMGB1 by Seraph-100 and Heparin Sepharose. Plasma samples from sepsis patients were incubated with 10 vol% of adsorbent for 60 min at 37 °C and the remaining concentrations for (a) PF4, (b) histones/nucleosomes and (c) HMGB1 were determined. Plasma samples incubated without adsorbent served as controls. Data are presented as mean ± standard deviation (Kruskal–Wallis test followed by Dunn’s multiple comparisons; n = 9 for nucleosomes, n = 10 for HMGB1 and PF4; * p < 0.05, **** p < 0.0001). Results for histones/nucleosomes are given as arbitrary units (AU), which were calculated as a ratio of the highest optical density (OD) value and the OD value of individual samples.