Table 2.
Antibodies and fluorochrome conjugates used for flow cytometry and confocal microscopy.
| Flow Cytometry | ||||||
| Antigen | Origin | Clone | Marker for | Fluorochrome | Abbreviation | Supplier |
| CD41 | Mouse | P2 | Platelets | Phycoerythrin Cyanin 7 | PC7 | Beckman Coulter |
| CD62P | Mouse | CLB Thromb6 | Activated platelets | Fluorescein Isothiocyanate | FITC | Beckman Coulter |
| CD63 | Mouse | H5C6 | Activated platelets | Alexa Fluor 647 | AF647 | BioLegend |
| PF4 | Mouse | # 170138 | Activated platelets | Phycoerythrin | PE | R&D Systems |
| Anx5 | - | - | Phosphatidyl-serine | Allophyco-cyanin | APC | BD Biosciences |
| Confocal Microscopy | ||||||
| Antigen | Origin | Clone | Marker for | Fluorochrome | Abbreviation | Supplier |
| PF4 | Mouse | D7 | Activated platelets | - | - | Santa Cruz Biotechnology |
| Mouse IgG | Goat | Polyclonal | - | AlexaFluor 488 | AF488 | Jackson Immunoresearch |