Figure 6.

Effects of the γ-Eno peptide on proliferation of GBM cells, GSCs, and GBM-associated cells. GBM cells NIB140, GSCs NCH421k (A), as well as differentiated macrophage THP-1 cells (B) and BV-2 microglial cells (C) exposed to the NIB140- and NCH421k-conditioned media were treated with increasing concentrations of the γ-enolase peptide, C-terminal 30-amino-acid sequence of human brain γ-enolase (γ-Eno). Cell proliferation was evaluated using CFSE staining and flow cytometry. The control means a blank culture GBM cell/GSC medium without addition of γ-Eno (A). The control medium is a blank GBM cell/GSC medium without soluble molecules secreted from GBM cells/GSCs and without addition of γ-Eno (B,C). The data are presented as the means $\pm$SEM. * p < 0.05, ** p < 0.01.