Figure 4.

Gzm protein expression within hematopoietic cells as determined by mass spectrometry. Using the Immunological proteomic resource (ImmPRes), an open access proteomic database of murine immune cell populations generated using large scale quantitative mass-spectrometry, we examined Gzm expression within hematopoietic cells. Identification of protein expression utilized data-dependent acquisition tandem mass spectrometry. Copy number refers to the average protein concentration per cell. From this dataset we have found that GzmB seems to have the most diverse expression within different cell types examined and is often the most abundant Gzm. Other Gzms have more unique expression patterns and are only expressed on a smaller subset of the immune cells examined. Critically, T cell expression of most Gzms seems to be dependent on their activation status.