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. 2022 Feb 2;11(3):799. doi: 10.3390/jcm11030799

Figure 6.

Figure 6

The effect of EPO on mRNA expression and collagen production in mammary fibroblasts. Messenger RNA expression of cancer-associated genes (A) and genes involved in extracellular matrix regulation and collagen production (B) measured by RT-PCR in human mammary fibroblasts following treatment with 2.5 μg/mL of EPO for 72 h. mRNA expression normalised to HPRT1 expression and presented as relative expression where the average for untreated controls is 1. Collagen production was measured using collagen 1 ELISA (C) and insoluble collagen measured by sirius red staining (D). Data presented as mean + SEM with statistical analysis performed using a linear mixed-effects model Tukey’s post hoc comparison. Statistical significance indicated by * when p < 0.05.