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. 2022 Feb 6;23(3):1834. doi: 10.3390/ijms23031834

Figure 3.

Figure 3

Inhibition of CENTA hydrolysis by metallo-β-lactamase NDM-1 in the presence of unithiol and zinc ions. Experimental conditions: initial concentrations of the NDM-1 (20 nM), CENTA (50 μM), 1—no unithiol and zinc ions; 1a—zinc ions (50 μM); 2—unithiol (20 μM); 2a—unithiol (20 μM) and zinc ions (50 μM); 3—unithiol (50 μM); 3a—unithiol (50 μM) and zinc ions (50 μM). The hydrolysis product accumulation is monitored in the 50 mM sodium phosphate buffer, pH 7.0 at 25 °C by continuous recording of the absorbance at 405 nm.