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. 2022 Feb 7;23(3):1881. doi: 10.3390/ijms23031881

Figure 2.

Figure 2

Cryopreservation alters the relative distribution of leukocyte populations. (A) Relative number of gated monocytes, T cells, B cells, and NK cells before and after cryopreservation analyzed by flow cytometry. Cell amounts are given as % live CD45+ cells. (B) Correlation plots of relative cell amounts of monocytes, T cells, B cells, and NK cells before and after cryopreservation. Correlation was analyzed by linear regression. (C) Analysis of dead monocytes, T cells, B cells, and NK cells before and after cryopreservation. Please notice the different scales on the y-axis of the two left and two right panels. Gated cells staining positive for intracellular SytoxGreen are shown relative to all CD45+ cells measured by flow cytometry. * p < 0.05; ** p < 0.01; *** p < 0.001; † = significantly different from all other conditions; § = significantly different from other conditions except the ones indicated with “ns”. (n = 15 individual human donors).