Figure 4.

A 1-h resting period after thawing does not dramatically alter leukocyte population amounts. (A) Comparison of the relative amounts of monocytes, T cells, B cells, and NK cells without or with a 1-h resting period after cryopreservation before analysis using flow cytometry. The dotted lines labelled “Fresh” indicate the mean values of the fresh samples for each gated cell population. (B) Comparison of the relative distribution of monocyte subsets without or with a 1-h resting period after thawing before analysis using flow cytometry. The dotted lines labelled “Fresh” indicate the mean values of the fresh samples for each gated cell subset. * p < 0.05; ** p < 0.01; *** p < 0.001. (n = 15 individual human donors).