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. 2022 Feb 7;23(3):1881. doi: 10.3390/ijms23031881

Figure 6.

Figure 6

Cryopreservation affects the activatability of the CD11b surface integrin. (A) Representative flow cytometry overlays of the activated CD11b fluorescence of the gated intermediate monocyte subset for the fresh condition and cryopreservation media. Dotted lines represent the unstimulated monocytes, whereas filled lines represent the LPS-stimulated monocytes. (B) Median fluorescence intensity (MFI) of activated CD11b expression of the classical monocyte subset measured by flow cytometry for unstimulated and LPS-stimulated monocytes. (C) Delta fresh MFI values of cryopreserved classical monocytes either unstimulated or stimulated with LPS. Negative values indicate lower MFI compared to the individual fresh condition, and positive values indicate higher MFI compared to the individual fresh condition. (D) Median fluorescence intensity (MFI) of activated CD11b expression of the intermediate monocyte subset measured by flow cytometry for unstimulated and LPS-stimulated monocytes. (E) Delta fresh MFI values of cryopreserved intermediate monocytes either unstimulated or stimulated with LPS. Negative values indicate lower MFI compared to the individual fresh condition, and positive values indicate higher MFI compared to the individual fresh condition. (F) Median fluorescence intensity (MFI) of activated CD11b expression of the non-classical monocyte subset measured by flow cytometry for unstimulated and LPS-stimulated monocytes. (G) Delta fresh MFI values of cryopreserved non-classical monocytes either unstimulated or stimulated with LPS. Negative values indicate lower MFI compared to the individual fresh condition, and positive values indicate higher MFI compared to the individual fresh condition. * p < 0.05; ** p < 0.01; *** p < 0.001; § = significantly different from other conditions except the ones indicated with “ns”. (n = 15 individual human donors).